D1190
Sigma
DT Diaphorase from rat
~90% (SDS-PAGE), lyophilized powder, recombinant, expressed in Escherichia coli
| Synonym: | DTD, NQO1, Quinone reductase |
| Enzyme Commission (EC) Number: | 1.6.99.2 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00130947 |
Description
| Biochem/physiol Actions | Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules. |
| Other Notes | Catalytic activity is different from human source DT diaphorase |
| Unit Definition | One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C. |
| Physical form | Lyophilized powder containing approx. 5% protein balance lactose and PBS buffer salts |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ~90% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | monomer mol wt 31000 |
| Gene Information | rat ... Nqo1(24314) |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
Related Products
References
| reference | Chen, S., et al., Molecular Basis of the Catalytic Differences among DT-Diaphorase of Human, Rat, and Mouse. J. Biol. Chem. 272, 1437-39, (1997) |
